mcp 1 levels  (Elabscience Biotechnology)

Journal: Journal of molecular and cellular cardiology

Article Title: Mouse model of post-traumatic stress disorder negatively impacts cardiac homeostasis

doi: 10.1016/j.yjmcc.2025.01.011

Figure Lengend Snippet: A) Male PTSD-like mice had an increased trend ( p = 0.0542) in macrophages (Mac-3) in the left ventricle at 4-weeks post-IFS. No differences were observed in the female groups. Male control n = 3; Female control n = 4; Male NR n = 6; Female NR n = 7; Male PTSD-like n = 6; Female PTSD-like n = 5. B) No differences between phenotypes at 4-weeks post-IFS were observed in circulating levels of CCL2. Male control n = 4; Female control n = 4; Male NR n = 3; Female NR n = 6; Male PTSD-like n = 6; Female PTSD-like n = 4. C) Male and female NR and PTSD-like mice had decreased percent area of Ccr2 mRNA expression compared to respective controls. No significant changes were seen in the amounts of Ccr2 + CD206 mRNA colocalization but female NR mice had an increase in Ccr2 + CD86 mRNA colocalization compared to female controls at 4-weeks post-IFS. Male control n = 3; Female control n = 4; Male NR n = 5; Female NR n = 7; Male PTSD-like n = 6; Female PTSD-like n = 5. Multiple group comparisons were analyzed by two-way ANOVA with Bonferroni posttest.

Article Snippet: Assays for MMP-9 activity (Enzo, BML-AK410, 1:5 dilution of plasma, no dilution of insoluble protein fraction of the LV), circulating CCL2 levels (R&D Systems, MJE00B, 1:10 dilution), and circulating cardiac troponin I (Novus Biologicals, NBP3-00456, 1:2 dilution) were performed according to manufacturer instructions.

Techniques: Control, Expressing

Journal: The Journal of Clinical Investigation

Article Title: Induced clustering of SHP2-depleted tumor cells in vascular islands restores sensitivity to MEK/ERK inhibition

doi: 10.1172/JCI181609

Figure Lengend Snippet: ( A ) Expression levels of Il1a , ccl2 , and ccl3 in sorted populations of EGFP + B16F10 cells, EGFP – VE-cadherin + endothelial cells, and EGFP – CD45 + hematopoietic cells as measured by qPCR. The results are expressed as the relative mean ± SEM of Gapdh mRNA levels ( n = 3 experiments). ( B and C ) Representative images (scale bar: 50 μm) ( B ) showing CD45 + cell infiltrates (green, indicated by arrowheads) in control and shSHP2-silenced B16F10 tumors and quantification ( C ). Gray circles: percentage of CD45 + area/unit of tumor area; colored dots: mean/tumor ( n = 5/group). ** P < 0.01, by 2-tailed Student’s t test. ( D and E ) Representative images (scale bar: 50 μm) ( D ) showing F4/80 (green) macrophage infiltration in control and shSHP2-silenced B16F10 tumors (indicated by arrowheads) and quantification ( E ). Gray circles: percentage of F4/80 + area/unit of tumor area; colored dots: mean/tumor ( n = 5/group). ** P < 0.01, by 2-tailed Student’s t test. ( F ) Proposed model of downstream effects from SHP2 silencing in B16F10 melanoma cells leading to the formation of vascular tumor islands and surrounding tumor cell death. Results in panels A , C and E are presented as means± SEM.

Article Snippet: Tumor CCL2 levels were measured with the Mouse CCL2 DuoSet ELISA kit (R&D Systems, mLMJE00B), and the colorimetric reaction was measured at 450 nm using a microplate reader (Imgen Technologies, BMG LABTECH).

Techniques: Expressing, Control

Journal: The Journal of Biological Chemistry

Article Title: Cardioprotective properties of OMT-28, a synthetic analog of omega-3 epoxyeicosanoids

doi: 10.1016/j.jbc.2024.107372

Figure Lengend Snippet: OMT-28 limits LPS-induced inflammation in cardiac cells. Analysis of several inflammatory markers expressed by cardiac cells exposed to 24 h LPS (1 μg/ml) and treated with either vehicle, 19,20-EDP or OMT-28. A , ELISA quantification of TNFα expression in HL-1 treated with either vehicle, 19,20-EDP or OMT-28 (10 nM, 100 nM, and 1 μM) following LPS insult. Analysis of inflammatory markers in NRCM. B , TNFα expression, C , MCP-1 expression, D , TGF-β expression, and E , NF-κβ DNA-binding activity. Values represent mean ± SEM, data were obtained by analyzing responses of three independent cell preparations and using at least three technical replicas, p < 0.05 statistically significant, one-way ANOVA, Bonferroni post hoc test, ∗ versus vehicle control in PBS treated cells: # versus vehicle control in LPS-treated cells.

Article Snippet: Cell culture medium was centrifuged for 5 min at 5000 g , and supernatants were analyzed by ELISA for TNFa (Abcam, ab100747) and MCP-1 levels (Abcam, ab208979).

Techniques: Enzyme-linked Immunosorbent Assay, Expressing, Binding Assay, Activity Assay